Preparation and Characterization of Crystals of Prulifloxacin

Objective: Aim of this present study was to prepare and characterize the different polymorphs of Prulifloxacin using Powder X-Ray Diffraction analysis (PXRD), Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared Spectrometry (FTIR) and dissolution studies. Methods: Polymorphs of Prulifloxacin were prepared by cooling crystallization method using various solvents and they exhibited three different polymorphic forms that polymorph 1 (P-1), polymorph 2 (P-2) and polymorph 3 (P-3) with acetonitrile, acetone and dichloromethane. Results: Rod shaped crystal obtained from acetone (P-2) showed the highest percentage drug release in dissolution study than other crystals due to smaller particle size. P-1 form showed higher melting point in thermogram due to more stability. Melting temperatures obtained from DSC graph indicate the existence of different crystal forms. Significant differences were found in FTIR studies. Appearance and disappearance of specific peaks on the PXRD pattern reveal that the formation of newer crystal forms. Conclusion: It was concluded that the Prulifloxacin existed three polymorphic forms and they showed highest solubility and percentage drug release than amorphous form.